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The effect of ultrasound stimulation versus bioreactors on neocartilage formation in tissue engineering scaffolds seeded with human chondrocytes in vitro.

Hsu SH, Kuo CC, Whu SW, Lin CH, Tsai CL

Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan, ROC. shhsu@nchu.edu.tw <shhsu@nchu.edu.tw>

Pulsed ultrasound (1 MHz, 67 mW/cm(2) Ispta, and 10 min/day) promoted cell proliferation and matrix deposition in low-density 2D ( approximately 6 x 10(3)cells/cm(2)) as well as 3D ( approximately 4 x 10(6)cells/cm(3)) chondrocyte cultures. The beneficial effect of ultrasound on neocartilage formation only last 28 days, shorter than that of bioreactors.

Published 30 August 2006 in Biomol Eng, 23(5): 259-64.
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